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KMID : 0369919880030000067
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Journal of Pharmaceutical Sciences (C.B.N.)
1988 Volume.3 No. 0 p.67 ~ p.72
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An Improved HPLC Assay Using Hg/Au Electrochemical Detector for S-2-(3-aminopropylamino) ethylphosphorothioate and S-2(3-methylaminopropylamino) ethylphosphorothioate in Human Plasma
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Han Kun
Emil T. Lin
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Abstract
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WR 2721 (S-243-aminopropylanrninoethylphosphorothioate) is a radioprotective drug that is now undergoing clinical trials in the United States and Japan. a liquid chromatographic electrochemical method for the determination of WR 2721 an WR 3689 [S-243-methylaminopropylamino)ethylphorothioatel in human plasma was developed in this study. This method includes the use of a Hg/Au electrochemical detector and a cyano column for the direct measurement of WR 2721 and WR 3689 in plasma. An analog of WR 2721, WR 149846-was used as an internal standard. WR 2721 and WR 3689 could be well separated from the solvent front, with a mobile phase of acetonitrile-water (20:80), O.1M acetic acid and 1.2 mM sodium octane sulfonate. This method was shown to be precise. Both intra-day and inter-day results were within 10% CV. Also, sample preparation was fairly simple. Since WR 2721 and WR 3689 were unstable at room temperature, it was essential to use an automatic sample processor with a refrigerator, especially for carrying out routine analyses.
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